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KMID : 0360220140550010110
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Journal of the Korean Ophthalmological Society
2014 Volume.55 No. 1 p.110 ~ p.118
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The Effect of RGP Lens and Reverse Geometry Lens on Redistribution of Corneal Epithelial Cell in Rabbit
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Jeong Yong-Jin
Lee Ha-Bum
Park Sung-Pyo
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Abstract
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Purpose: To investigate the migration and redistribution of rabbit corneal epithelial cells when wearing reverse geometry lens (RGL) or rigid gas permeable lens (RGP).
Methods: In 30 rabbits, the right eyes were fitted with either RGL or RGP and the left eyes were untreated to serve as controls. The rabbits were sacrificed at 1, 3, 7, 10 and 14 days after lens fitting. The central and peripheral corneal thicknesses were measured by microscope and the ratio of right to left corneal thickness was calculated to evaluate the characteristics of change over time. By using the molecular probe 7-nitrobenz-2-ox-1,3-diazolylphallacidin (NBD phallacidin), the samples were examined with light microscope to determine the migration and redistribution of epithelial cells in the rabbit cornea.
Results: No consistent changes in the thickness of both central and peripheral corneal epithelium were found. The corneal epithelial cells of both eyes with RGL and RGP reacted positively to NBD phallacidin. The fluorescence was most increased at day 3 of sacrifice in RGL cases and at day 7 in RGP cases, and then decreased in both cases. The corneal epithelium of eyes with RGL exhibited marked increase in the intensity of fluorescence compared to the eyes with RGP.
Conclusions: The corneal epithelium with RGL showed the strongest intensity of NBD phallacidin fluorescence. This result suggests that wearing RGL may induce the migration and redistribution of corneal epithelial cells.
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KEYWORD
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Corneal epithelial cell, Redistribution, Reverse geometry lens, 7-nitrobenz-2-ox-1, 3-diazolylphallacidin (NBD phallacidin)
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